Primers for amplifying the hypervariable, male-transmitted COII-COI junction region in amblemine freshwater mussels (Bivalvia: Unionoidea: Ambleminae)
Freshwater bivalves in the superfamily Unionoidea possess distinct male (M)- and female (F)-transmitted mitochondrial DNA (mtDNA). The former evolves independently of and at a significantly faster rate than the latter. Thus, population genetic and phylogenetic analyses of M sequences facilitate the generation of independent estimates of genetic variation and evolutionary relationships which are often more robust than those provided by analyses of F sequences alone. However, M mtDNA's rapid substitution rate often renders polymerase chain reaction (PCR) amplification difficult with ‘universal’ primers. Herein, we report on three pairs of PCR primers that consistently amplify the hypervariable M COII-COI gene junction region in 25 bivalve genera (Unionoidea: Ambleminae).
Walker JM, AE Bogan, EA Bonfiglio, DC Campbell, AD Christian, JP Curole, JL Harris, RJ Wojtecki, & WR Hoeh. 2007. Primers for amplifying the hypervariable, male-transmitted COII-COI junction region in amblemine freshwater mussels (Bivalvia: Unionoidea: Ambleminae). Mol. Ecol. Notes 7(3):489-491. https://onlinelibrary.wiley.com/doi/10.1111/j.1471-8286.2006.01630.x